LOCUS       40924_23864       12840 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB755 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12840)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12840)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1852
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     protein_bind    1862..1986
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2023..2053
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2107..2763
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3108..3410
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3454..3578)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3604..3856
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3890..3906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3914..3930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3938..3968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3983..4004)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4040..4292)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4330..5625)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61292.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4330..5625)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5677..5856)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6362..6386)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6907..7695
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7944..8532
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8718..8858)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9202..9396)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9465..10535)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10967..11593)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gcctcctccg aggacgtcat
     1201 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     1261 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     1321 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
     1381 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     1441 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     1501 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     1561 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     1621 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
     1681 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
     1741 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
     1801 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg ccgctgttat
     1861 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     1921 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     1981 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2041 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     2101 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     2161 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     2221 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2281 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2341 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2401 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2461 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2521 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2581 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2641 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     2701 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2761 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     2821 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     2881 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     2941 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     3001 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     3061 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     3121 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     3181 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     3241 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     3301 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     3361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     3421 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     3481 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3541 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga taacctagag ctcgaatttc
     3601 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     3661 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     3721 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     3781 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     3841 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
     3901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     4021 cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     4081 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     4141 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     4201 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     4261 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgcctcgag tgacttctct
     4321 gattgcagac tatttatacc aaccagcaag aaagtgtcga agtatgaagc agaaggcgca
     4381 ggctagagcc tggaaaacga gaagatggat gcaaagagac ttttctgatt taggaccaaa
     4441 caacctcaag taaacattga cgagtgtccc atcttttgtg ccggtcagta atccggtagc
     4501 aggatcatac cttgggagac gatggcgcgg acatttcaca atcccagcaa gctgaggaag
     4561 cgataataga aggttcagaa cttgaggaag aaagaagatg aggagggttt cactgaagtg
     4621 acccagaata ccaacaactg ccatagtcat tccggcgaag actgtgtaag tgtctccaac
     4681 aaaaactgaa gaaggatacc aattgtaagc aagcattgcc aaggatgtgg ccatcagcgg
     4741 ttgagtaaga aatatcgaga aggcatgagc ttggtgatac tcgggatcaa cagatgctcc
     4801 gatttgcata acgttgtgta tcaggatagc agcggcaata acaactgttt gaccgatttc
     4861 aaggccattc agaccagcgt ggatgttaat agagtttgta caaaagacag caagtagccc
     4921 catatataac ttatatatcc ttcctagatt aagcacttcc aagccaatat aagcaacaag
     4981 aggttttggt ataacaatag ttgtatgacc agcataagcc atcaaaagtg gaagggtagc
     5041 aaaagatggc aggacaagtt ttactctcca aggcacatca aggacatcat ctacaaatcc
     5101 aagcaaaatc atgaagcaaa tagatgctag tgctgcatta tactccacaa gccaattaga
     5161 atcttcagtg aagttaaaat actgaaatat tatcgccacg atcaaaaaga caataccaac
     5221 aacaataccc aatgactctg gcactttaat atctccttga ggcgtgcctc ttttgttgat
     5281 atcaaaacca aacatgttac gtctcagaac gtatctggca gtaacaggaa tcattttgag
     5341 agtgacgaaa aacccaacca agctaagccc cgcgttaatt agaatcgatc gcttgagttc
     5401 attatcaacc ttgtagtgaa aacagagaag gtatagatag agcgaacaga gaagagtaga
     5461 gatcacgaag atcactccta atttcggcgg cgcgagacga aattcctcac cggaagcaga
     5521 aaccgtcttt cgggtcatct tctgctccga tggcgctgag ttgggttccg acgggtccgg
     5581 tttgttggga attgagatcg atgaagctct cttacgagct gccatatctt attgcccccc
     5641 gggatcagat ctggattgag agtgaatatg agactctaat tggataccga ggggaattta
     5701 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     5761 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     5821 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     5881 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct
     5941 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     6001 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     6061 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     6121 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     6181 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     6241 cttgcggcag cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg
     6301 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     6361 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     6421 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     6481 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     6541 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     6601 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     6661 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     6721 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     6781 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     6841 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     6901 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     6961 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     7021 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     7081 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     7141 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     7201 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     7261 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     7321 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     7381 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     7441 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     7501 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     7561 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     7621 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     7681 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     7741 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     7801 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     7861 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8701 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8761 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8821 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     8881 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8941 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     9001 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     9061 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     9121 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     9181 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     9241 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     9301 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     9361 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     9421 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     9481 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     9541 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     9601 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     9661 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     9721 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     9781 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     9841 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     9901 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     9961 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    10021 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    10081 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    10141 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    10201 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    10261 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    10321 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    10381 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    10441 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    10501 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    10561 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
    10621 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    10681 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    10741 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    10801 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    10861 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    10921 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    10981 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    11041 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    11101 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    11161 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    11221 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    11281 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    11341 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    11401 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    11461 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    11521 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    11581 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    11641 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    11701 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    11761 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    11821 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    11881 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    11941 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    12001 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    12061 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    12121 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    12181 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    12241 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    12301 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    12361 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    12421 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    12481 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    12541 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    12601 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    12661 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    12721 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    12781 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
